Micropropagation, callus induction and regeneration of ginger. Effective in vitro micropropagation and callus induction aid plant material. Callus induction was achieved from both the leaf disc and internodal explants from 10 to 15 days of incubation. The ability of callus formation depends on explant source and influenced by type of growth regulator in their concentrations and combinations in the growth medium. Hairy roots formed directly from the cut edges of leaf explants or via callus stage 8 days after inoculation with the bacterium. This study describes a protocol for in vitro callus induction and plant regeneration from leaf and stem explants of c.
Studies on the production of some important secondary. Exogenous application of auxin and cytokinin induces callus in various plant species. Higher concentrations of 2, 4d 3 mgl was more effective for callus induction on terminal buds and flower explants. Phytohormonal effect on in vitro callus induction of. Afolayanb w e have devised a procedure for in vitro plant regeneration, from seedderived callus, of arctotis arctotoides, a plant noted for its medicinal uses among the rural people of the eastern cape province. Callus induction and organogenesis from explants of aconitum heterophyllum medicinal plant author. International journal of innovations in engineering and science, vol. An aromatic medicinal plant article pdf available in americaneurasian journal of sustainable agriculture 51. The kinetics of the spatial and temporal distribution of hormonal and developmental meristem regulators has been investigated by microscopic livecell imaging. A broad range of dissimilarity was observed in rate of callus. Plants develop unorganized cell masses like callus and tumors in response to various biotic and abiotic stimuli. Data analysis showed a callus induction rate of 88.
Callus induction and shoot regeneration in ducrosia anethifolia an important threatened medicinal plant abstract ducrosia anethifolia is a threatened medicinal herb belongs to the family apiaceae. Phytochemicals are serving as a major source of pharmaceuticals, flavors, agrochemicals, colors, biopesticides and food additives. Phyllanthus debilis, ms medium, callus induction, plant growth regulators. Medicinal plants have long been used for health care purpose throughout the world since ancient times by ayurvedic. In vitro callus induction studies on abrus precatorius l. Profuse callus was formed on ms medium supplemented by bap 0. The study was carried to promote the synthesis of callus production from different explants such as leaf, node and internode from a traditional medicinal plant, mukia maderaspatana l. Plant regeneration from seedderived callus of arctotis. Among the treatments, vermicompost medium and vermicompost extract medium with coelomic fluid have shown maximum 100 per cent callus induction table 1, figure 1b. Root, hypocotyl, and cotyledon segments were cultured on murashige and skoog ms 1962 medium supplemented with either 2,4dichlorophenyoxyacetic acid 2,4d or naphathalene acetic acid naa, at 02 mg l. In vitro callus induction and plantlet regeneration of achyranthes aspera l.
Callus induction and elicitation of total phenolics in. The induction of callus is usually promoted by an auxin, especially 2,4d10. Original contribution callus induction and plant regeneration. Today several distinct chemicals derived from plants are important drugs currently used in one or more countries in the world. Medicinal plants used for the treatment of various skin disorders by a rural community in warud region of maharashtra. Medicinal plants are of great interest to the researchers in the field of. Msi medicinal use of the unique plant tinospora cordifolia. Apiaceae, a native medicinal plant in iran, is classified as a vulnerable species. Callus induction and plant regeneration from leaf explants. Generally, the callus fresh weight derived from young petiole was higher than that produced callus from young stem and apical meristem. In vitro callus induction and plant regeneration of argentea. In vitro characteristics of callus induction of bryonia laciniosa a medicinal plant dr. Pdf studies with callus induction of vitex negundo.
Studying of callus induction and plant regeneration of medicinal plants valeriana offinalis. Callus induction and somatic embryogenesis in stevia rebaudiana bertoni as a medicinal plant article pdf available in cellular and molecular biology noisylegrand, france 642. Micropropagation and callus induction of lantana camara l. In vitro callus induction and indirect organogenesis of. Figure 1 regenerated plants and calli formation on ms medium containing 2. The optimized callus induction occurred at the concentration of 1. In this experiment, an investigation was done to find out a suitable and standard protocol for callus induction of an indian traditional medicinal plant, convolvulus alsinoides linn. Invitro callus induction and shoot regeneration in. Shoot multiplication, root induction and callus formation were investigated. Arabidopsis and tobacco seed germination on plant callus induction medium. Discussion the highest percentage of callus induction for stem. Invitro callus induction and rosmarinic acid quantification in. In vitro callus induction and plantlet regeneration of.
Callus induction from leaf discs, 1 mgl iaa and 1 mgl bap. L1 had a similar effect on callus induction and callus induction were reduced by increasing amount of this hormone to 3. Invitro callus induction and shoot regeneration in ephedra a. High frequency regeneration of plants via callusmediated. Pdf callus induction and plant regeneration in dorem. Callus induction in coscinium fenestratum in the present study, c. Callus induction and somatic embryogenesis from leaf and. Received 10 th, may, 2014 received in revised form 17. Original article callus induction and shoot regeneration. It produced when explants are cultured on the appropriate solid medium, with both an auxin and a cytokinin in a correct conditions. In this article the effects of explants types, plants growth regulators and elicitors on callus induction and cell culture conditions were studied.
In vitro callus induction and plant regeneration of. Since ms medium supplemented with 2,4d gave better results in terms of per cent callus response, days required for callus induction and callus growth, effect of 2,4d in combination of bap 0. Callus induction and organogenesis from explants of. Generally speaking, an intermediate ratio of auxin and cytokinin promotes callus induction, while a high. In these plants, gradients of the phytohormone auxin in embryonic callus lead to the induction of stem cell formation by regulation of the pinformed1 pin1 protein. Callus induction rate and regeneration capacity of callus were greatly influenced by the genotype. In vitro callus induction and plant regeneration from. In the present study, the best explant and corresponding treatment for callus induction and somatic embryogenesis in this species were determined. Suitable callus induction was on ms medium supplemented with 0. Plants are a tremendous source for the discovery of new products of medicinal value for drug development.
Pdf in vitro callus induction and comparative antimicrobial. Arabidopsis and tobacco seed germination on plant callus. Harvest the roots of these seedlings after 1520 d of growth see note 4. Callus induction and organogenesis from explants of aconitum. Callus induction, proliferation, and plantlets regeneration. Pdf callus induction and somatic embryogenesis in stevia. The in vitropropagated medicinal plants furnish a ready.
Introduction tissue culture is the process whereby small pieces of living tissue explants are isolated an organism and grown aseptically for indefinite periods on a nutrient medium under controlled condition. Effect of plant hormones on callus induction of explant. Sep 27, 20 callus formed under in vitro culture conditions. In vitro callus induction and plant regeneration of celosia. The leaf segments were inoculated on ms medium supplemented with various concentrations 0. Effect of auxin and cytokinin combination ratio on callus induction.
In vitro propagation has been accomplished in many apiacean species, including centella asiatica 19, eringium foetidum 7, 12, cuminum cyminum l 3. Leaves and nodes were used as explants and cultured on different combinations. Callus formation in vitro and in nature callus formed under in vitro culture conditions exogenous application of auxin and cytokinin induces callus in various plant species. Afolayanb w e have devised a procedure for in vitro plant regeneration, from seed derived callus, of arctotis arctotoides, a plant noted for its medicinal uses among the.
Further, indirect organogenesis offers the induction of variability and thereby the improvement of the crops. In addition, evaluation of mean values showed that callus fresh weight was significantly concentration of growth regulators table 2. Callus it is an unspecialized, unorganized, growing and dividing mass of cells. Study of callus induction and cell culture to secondary. Lycium barbarum or commonly known as goji or wolfberry is a type of chinese medicinal herbs which has been consumed since ancient times. Callus induction and elicitation of total phenolics in callus. Callus induction and plant regeneration from leaf explants of. Place the roots into a petri dish, remove all liquid and cut the roots into small pieces. Since the historical discovery that the combination of two growthpromoting hormones, auxin and cytokinin, induces callus from plant explants in vitro, this experimental system has been used extensively in both basic research and horticultural applications. Fabaceae, in vitro, callus induction recalcitrant, tissue culture. In vitro characteristics of callus induction of bryonia.
Obtained callus when transferred to fresh nutrient medium d containing ms salts. Biotechnology applications of plant callus cultures. An efficient callus induction from phyllanthus debilis klein. Antimicrobial and antifungal activities of the essential oil of hyssop have been reported. An efficient transformation system for the medicinal and aromatic plant, pogostemon cablin benth was developed by using agropinetype agrobacterium rhizogenes atcc15834. Lia shooshtari kermanshahi, mansoor omidi, eslam majidi, mohammadreza naghavi, shamsali rezazadeh. Callus induction from leaves and internode segments of iv. Generally speaking, an intermediate ratio of auxin and cytokinin promotes callus induction, while a high ratio of auxintocytokinin or cytokinintoauxin induces root and shoot regeneration, respectively skoog and miller, 1957. Day of callus induction started from th to 37 day. Greenishreddishwhite compact and friable callus fig.
Plant regeneration from callus culture of clematis. High efficient protocol for callus induction and regeneration of a. In vitro callus induction and antimicrobial activities of. Effective in vitro micropropagation and callus induction aid plant material production for bioactive compound studies and plant resource conservation. Evidence from the traditional medicine and recent research. An in vitro regeneration protocol through stem callus culture has been standardized for the medicinal climber clematis gouriana. The highest frequency of leaf explant transformation by agrobacterium rhizogenes atcc15834. Original article callus induction and shoot regeneration in. The whole callus mass is taken out aseptically on a sterile petridish and should be divided into two or three pieces. Since the historical discovery that the combination of two growthpromoting hormones, auxin and cytokinin, induces callus from plant explants in vitro, this experimental system has been used extensively in both basic research and horticultural. Many of the drugs sold today are simple synthetic modifications or copies of the naturally obtained substances.
The best results were achieved on the following media. Well rooted plants were hardened in green house conditions and the survival rate is 100%. Stem explants were cultured on ms medium with different combination of plant growth regulators 2,4d and coconut water for callus induction. In plant culture, no two genotypes give similar response under a. As rhizome explants are underground part of ginger plants, the in vitro.
Formed and isolated callus tissue induction of organogenic callus and proliferation. Pdf studying of callus induction and plant regeneration of. Callus induction and elicitation of total phenolics in callus cell suspension culture of celastrus paniculatus willd, an endangered medicinal plant in india. Paper open access the effect of plant growth regulators. Place the flasks on a rotary shaker at 120 rpm using 16 h light and 8 h dark period at 25oc.
Effect of plant hormones on callus induction of explant types. Plant regeneration from seedderived callus of arctotis arctotoides, a medicinal herb of the family asteraceae p. Due to its anti inflammatory properties, ginger is used as a medicinal plant to treat a wide. Callus induction and plant regeneration in dorem ammoniacum d. Rapid invitro regeneration of an important medicinal and. The highest frequency of leaf explant transformation. Induction of hairy roots and plant regeneration from the. Rapid invitro regeneration of an important medicinal and an. To study callus induction from different explants internode, leaf, root and in vitro plantlets propagation from medicinally important plant achyranthes aspera l. Pdf callus induction of selected malaysian medicinal plants. Pdf studying of callus induction and plant regeneration. Jun 23, 2011 an efficient transformation system for the medicinal and aromatic plant, pogostemon cablin benth was developed by using agropinetype agrobacterium rhizogenes atcc15834. The explant induced callus on msmedium supplemented with 0. Results in table 1 showed that among all concentrations of naa, 1 mgl gave the highest callus induction from hypocotyls explants, while 5.